| Status: |
Public |
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| Title: |
Use of glycan array slides to validate the glycan-binding specificities of polyclonal IgY antibodies raised in chickens by inoculation with virus-glycan conjugates |
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| Glycan Array Version: |
PA_v1 |
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| Protocol Id: |
Printed Array Screening |
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| Replicate Number: |
1 |
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| Result Nature: |
Data |
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| Experiment Date [mm/dd/yyyy]: |
05/16/2006 |
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| Supplying Investigator: |
M.G. Finn |
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| Assay Information: |
The dilution buffer I used was PBS + 0.05% Tween20 and 0.3%BSA.
The wash buffers were:
1) PBS + 0.05% Tween20
2) PBS
3) Distilled H2O
Diluted the primary antibodies to 0.15 mg/ml
Diluted the secondary antibody anti-IgY 1:50 |
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| Annotation: |
Chicken polyclonal tri-Tri-LacNAc-IgY. Affinity purified on LacNAc-agarose column |
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