To screen Mouse Transgenics Core F generated glycosyltransferase KO mice in Core E and Core C.
CFG Member?
Yes subgroups :2 :Glycans in immune recognition and function
,6 :Glycan synthesis and microarrays
Request Details
Requester First Name:
Bo
Requester Last Name:
Ma
Head of Lab. First Name:
James
Head of Lab. Last Name:
Paulson
Assigned First Name:
James
Assigned Last Name:
Paulson
Requester Email:
jpaulson@scripps.edu
Requester Interest:
Dr. Paulson's group investigates the roles of carbohydrate-binding proteins involved in immune regulation and human disease, through their interaction with carbohydrate groups expressed on cell surface glycoconjugates. This laboratory is currently focused on how sialoside ligands modulate Siglec functions and on understanding the molecular basis of glycosylation changes following differentiation and activation of leukocytes.
Request Date:
06/29/2010
Institution:
On behalf of Core F
Comments:
This request generated for Core E and Core C to upload data per NIGMS mandate
CFG Core:
C2
Amount Requested:
Experiment to be conducted:
The purpose of this request is to respond to the NIGMS mandate to screen Core F generated glycosyltransferase KO mice in Core E and Core C.
The goal of this request is to screen several tissues for gene expression changes in ST6GalNAc2 KO mice and B4GalNT1/B4GalNT2 double KO mice relative to C75 Wild Type controls. Tissues for Core E analysis were selected by mining public databases including the NIH GEO database for tissues that expressed the KO genes at moderate to high levels. Results from Core E gene expression analysis is then used to help Core C select tissues to follow up with glycan analysis on in each of the KO mice.
Within Scope of Consortium:
Y
Specific aims being addressed:
* 1. Define the specificity and affinity for carbohydrate ligands
* 2. Establish the cell types involved in communication
Comments By Investigator
This request was intiated on behalf of Core F to place the data requested by NIGMS for this mouse strain. No publication will be initiated by this investigator, but others may use the data.